Study on the decay mechanism and preservative tech

2022-09-30
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Study on the decay mechanism and preservative and fresh-keeping technology of Chestnut (III)

2.2 correlation between the enzyme activity of rot causing microorganisms and temperature and pH value

2.2.1 the cellulase and amylase activities of Pseudomonas aeruginosa under different temperatures and pH values

the cellulase and amylase activities of Pseudomonas aeruginosa were measured at different temperatures, and the results are shown in Table 4 It can be seen from table 4 that the activity of cellulase and amylase of Pseudomonas aeruginosa is not significantly affected by temperature. At 25 ℃, the activity of cellulase and amylase of Pseudomonas aeruginosa is the largest, and the activity of cellulase and amylase of Pseudomonas aeruginosa is the lowest at 5 ℃ Cellulase activity was slightly higher at 15 ~ 25 ℃, while amylase activity was higher at 25 ~ 40 ℃

Table 4 cellulase and amylase activities of Pseudomonas aeruginosa at different temperatures

enzyme activity of the project at different temperatures 40 ℃ 35 ℃ 30 ℃ 25 ℃ 20 ℃ 15 ℃ 10 ℃ 5 ℃ cellulase activity/( μ- 1H-1) 27.2528.2931.5232.9132.2431.2425.3516.53 amylase activity/( μ- 1min-1) 39.5540.7443.2956.9736.9435.6536.5024.61

the results of determining the cellulase and amylase activities of Pseudomonas aeruginosa at different pH values are shown in Table 5 It can be seen from table 5 that the cellulase activity of Pseudomonas aeruginosa is relatively large between pH 4.2 and 6.2 when a single set of equipment reaches 5000 tons/year, and is the highest at pH 5.2, which is conducive to enzymatic reaction under acidic conditions Different pH values have influence on the amylase activity of Pseudomonas aeruginosa, and the enzyme activity changes greatly at different pH values The pH value between 6.7 and 7.2 is conducive to the degradation of starch Table 5 cellulase and amylase activities of Pseudomonas aeruginosa under different pH values

project enzyme activity under different pH values 9.28.27 can be divided into manual, full-automatic and microcomputer controlled models 26.25.24.23.22.2 cellulase activity/( μ- 1H-1) 28.8633.1044.5948.4453.4948.8436.4339.70 amylase activity/( μ- 1min-1) 58.50152.0188.1196.4164.1158.8149.0138.0

2.2.2 the cellulase and amylase activities of Chaetomium polymicum under different temperatures and pH values. The results of cellulase and amylase activities of Chaetomium polymicum under different temperatures are shown in Table 6 It can be seen from table 6 that the cellulase and amylase of Chaetomium polymicum in the temperature range of 5 ~ 40 ℃ are not significantly affected by temperature, and the data measured at each temperature are relatively close, which may be because the conformation of cellulase and amylase of Chaetomium polymicum has little change under the condition of 5 ~ 40 ℃, and the enzyme activity does not change

table 6 cellulase and amylase activities of Chaetomium multiflorum under different temperatures

enzyme activity under different temperature conditions of the project 40 ℃ 35 ℃ 30 ℃ 25 ℃ 20 ℃ 15 ℃ 10 ℃ 5 ℃ cellulase activity/( μ- 1H-1) 17.5926.7523.7832.0021.6929.0523.9621.38 amylase activity/( μ- 1s-1) 41.5336.8943.3839.8634.2637.0036.6435.89

the results of determining the cellulase and amylase activities of Chaetomium polybotryi under different pH values are shown in Table 7. From table 7, it can be seen that the cellulase and amylase activities are the largest when the pH value is 4.2, the cellulase activity is relatively large under strong acidic conditions, and the amylase activity is relatively large when the pH value is between 4.2 and 5.2, The results showed that pH had a significant effect on the activities of cellulase and amylase of Chaetomium polybotryi, which may be caused by the influence of pH in the crude extract of the enzyme, or the influence of pH on the active center of the enzyme

table 7 cellulase and amylase activities of Chaetomium multiflorum under different pH values

enzyme activity under different pH values of the project 9.28.27.26.25.24.23.22.2 cellulase activity/( μ- 1

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